brightfield microscopy keyence  (KEYENCE)

Journal: eLife

Article Title: A single cysteine residue in vimentin regulates long non-coding RNA XIST to suppress epithelial–mesenchymal transition and stemness in breast cancer

doi: 10.7554/eLife.104191

Figure Lengend Snippet: ( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in brightfield (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).

Article Snippet: Colour development was achieved using 3, 3’-diaminobenzidine (DAB) substrate, and slides were lightly counterstained with Harris haematoxylin, dehydrated, mounted in Permount, and imaged by brightfield microscopy (Keyence Corporation, Itasca, IL).

Techniques: Expressing, Staining, Software, CyQUANT Assay, Cell Adhesion Assay, Migration, Imaging

Journal: Acta Neuropathologica Communications

Article Title: Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s disease

doi: 10.1186/s40478-021-01145-2

Figure Lengend Snippet: Element maps in human SN nigrosome 1 in controls (Co) and patients with PD, obtained by µPIXE. Brightfield microscope images (top row) show the immunocytochemical reaction for cell-type specific antigens providing the basis for definition of regions of interest for the subsequent PIXE imaging (below). The ultrapure nickel-enhancement of the immunoreaction allows to allocate the µPIXE element signals to specific cell types. The iron map was used to quantify the local concentrations of iron in different cell types (see Fig. ). Scale bar top left 20 µm applies to all images

Article Snippet: Brightfield microscopy (Keyence BZ 9000) was performed before the μPIXE analysis for orientation, selection and documentation of the regions to be examined.

Techniques: Microscopy, Imaging

Journal: Acta Neuropathologica Communications

Article Title: Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s disease

doi: 10.1186/s40478-021-01145-2

Figure Lengend Snippet: Oligodendroglia activation and degenerative change of myelin in nigrosome 1 of controls (Co, a , b ) and PD ( c , d ) patients. Brightfield microscopy of myelin basic protein (MBP) shows a dense and rather homogeneous network of myelinated fibres in controls ( a ). In PD, myelinated fibres appear less dense ( c ). Additionally, in PD an increase of anti-Olig2 positive oligodendrocytes is visible (arrows, d ). Scale bar 50 µm

Article Snippet: Brightfield microscopy (Keyence BZ 9000) was performed before the μPIXE analysis for orientation, selection and documentation of the regions to be examined.

Techniques: Activation Assay, Microscopy